Device

Part:BBa_K5466023:Design

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-23)


Constitutive expression of AFB1 detection platform


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3825
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed this part to demonstrate how IGG6 reduces the size of genetic constructs when compared to BBa_K5466021 and BBa_K5466022, both of which are required to express the AFB1 detection platform.

BBa_K5466021: 2316 bp

BBa_K5466022: 3242 bp

BBa_K5466023 (using IGG6): 4647 bp

By utilizing IGG6, the total construct size is reduced by approximately 1000 bp compared to the combined 5558 bp of BBa_K5466021 and BBa_K5466022 required for the same function.

Source

Source in each individual part.