![](https://parts.igem.org/images/partbypart/icon_device.png)
Device
Part:BBa_K5466023:Design
Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán Group: iGEM24_UMA-MALAGA (2024-09-23)
Constitutive expression of AFB1 detection platform
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3825
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed this part to demonstrate how IGG6 reduces the size of genetic constructs when compared to BBa_K5466021 and BBa_K5466022, both of which are required to express the AFB1 detection platform.
BBa_K5466021: 2316 bp
BBa_K5466022: 3242 bp
BBa_K5466023 (using IGG6): 4647 bp
By utilizing IGG6, the total construct size is reduced by approximately 1000 bp compared to the combined 5558 bp of BBa_K5466021 and BBa_K5466022 required for the same function.
Source
Source in each individual part.